MIXED PAYLOAD CONTAINING POLYMERS AND USE THEREOF

摘要

The invention provides methods for delivery of payloads to targets in samples using mixed payload polymers. The invention further provides reagents and kits for practicing the methods of the invention. The invention also provides methods for preparation of reagents for use in the methods of the invention.

主权项

1. A method of delivering a payload in a mixed payload polymer to a target molecule, the method comprising,
contacting the target molecule with a bispecific ligand comprising a first binding site and a second binding site wherein the first binding site binds specifically to a payload and the second binding site binds specifically to the target which is not the payload; contacting the bispecific ligand with a mixed payload polymer comprising a tether molecule linked to two or more distinct payload molecules, wherein the distinct payload molecules comprise a distinct payload molecule that is the smallest payload molecule and at least one distinct payload molecule that is not the smallest payload molecule, wherein the bispecific ligand does not bind specifically to the smallest payload molecule; wherein the payload is delivered to the target molecule, and a) the detection sensitivity of a molecule for detection is 10−15 g/ml or less, and the molecule for detection is the target or is bound by the target, b) the payload molecules attached to the tether have a molecular weight of at least 10 kDa, c) at least 2-fold more payload is delivered to the target site using a bispecific ligand that binds to a payload molecule that is not the smallest payload molecule as compared to a bispecific ligand that binds to a payload molecule that is the smallest payload molecule, or d) the payload molecules attached to the tether have at least 50% of the activity of a molar equivalent of payload molecules not attached to a tether.