摘要 |
The present invention relates to a polypeptide having proline-specific endoprotease activity, wherein the polypeptide has less than 70% residual activity when the polypeptide has been kept at a temperature of 65°C for 15 min. The invention further relates to a polypeptide having proline-specific endoprotease activity comprising an amino acid sequence according to SEQ ID NO: 1, wherein SEQ ID NO: 1 comprises at least one amino acid substitution selected from the group consisting of M144L, S398F, V460A, I204F, Y205N, F279S, F263Y, P377A, L470P, and P477A, a nucleic acid encoding a polypeptide having proline-specific endoprotease activity, a method of making a variant polypeptide having proline-specific endoprotease activity, a recombinant host cell and a method of producing the polypeptide and a process for the preparation of a food or feed product wherein the polypeptide is used. |
申请人 |
DSM IP ASSETS B.V. |
发明人 |
LAAN, VAN DER, JAN METSKE;VONDERVOORT, VAN DE, PETER JOZEF IDA;CHRISTIS, CHANTAL;SPAANS, MARTINE;BRUINE-PAULUS, DE, ANGELA |