GENERATION OF LIBRARY OF SOLUBLE RANDOM POLYPEPTIDES LINKED TO mRNA

摘要

Methods and compositions are provided for producing libraries of soluble random polypeptides. In the methods, the fraction of hydrophilic residues in the polypeptide is controlled so as to maintain the solubility of the polypeptide constructs.

主权项

1. A method of producing a library of soluble polypeptides linked to polynucleotides encoding the polypeptides, comprising:
synthesizing a plurality of polynucleotides; producing encoded polypeptides from the polynucleotides; and linking each polypeptide to the polynucleotide that encodes it;wherein a proportion of hydrophilic or surface compatible amino acid residues in each polypeptide is selected so that the polypeptide will be soluble.