| 摘要 |
FIELD: chemistry.SUBSTANCE: method of obtaining octagalacturonide includes acid hydrolysis of pectin with the following separation of liquid phase from insoluble pectin residue and separation of octagalacturonide from it. As pectin, low-etherified pectin with degree of etherification not higher than 30% is applied. Acid hydrolysis is carried out in 0.1-0.2 mole/l solution of mineral acid at temperature 70-100°C until concentration of liquid phase not lower than 0.25 mg/ml is obtained. To separate octagalacturonide from liquid phase it is neutralised to pH not lower than 4.0. Then ethanol is added with constant mixing to concentration 12-19 vol %, after that, sediment of octagalacturonide is separated from liquid phase and dried. To obtain product of higher purity before drying sediment of octagalacturonide is washed with solution, which contains 0.1-0.5 mole/l of sodium chloride and 12-19 vol % of ethanol, and then washed with 70-85 vol % solution of ethanol.EFFECT: invention makes it possible to obtain octagalacturonide with high degree of purity from pectin.2 cl, 8 ex |
