发明名称 |
BI-DIRECTIONAL SEQUENCING COMPOSITIONS AND METHODS |
摘要 |
In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis. |
申请公布号 |
US2015353994(A1) |
申请公布日期 |
2015.12.10 |
申请号 |
US201514832772 |
申请日期 |
2015.08.21 |
申请人 |
LIFE TECHNOLOGIES CORPORATION |
发明人 |
MYERS Jason;CHEN Zhoutao;DRESSMAN Devin;NIKIFOROV Theo |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
1. A method for generating single-stranded nucleic acids, comprising:
a) hybridizing a first primer to a nucleic acid molecule; b) extending the first primer via polymerization under polymerization conditions, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid molecule; c) introducing a nick into a portion of the nucleic acid molecule that is hybridized to the extended first primer product, where the nick includes a free 5′ end and a free 3′ end in the nucleic acid molecule; and d) degrading the nucleic acid molecule from the free 5′ end of the nick using a degrading agent, thereby generating a single-stranded extended primer product which remains hybridized to an undegraded portion of the nucleic acid molecule. |
地址 |
Carlsbad CA US |