发明名称 |
QUANTIFICATION OF ADAPTIVE IMMUNE CELL GENOMES IN A COMPLEX MIXTURE OF CELLS |
摘要 |
A relative representation of adaptive immune cells in a biological sample is quantified using multiplex PCR and sequencing of adaptive immune cells, control genes, and synthetic template molecules. Disclosed herein are methods for quantifying a number of adaptive immune cells in a biological sample, and methods for quantifying a relative representation of adaptive immune cells in a biological sample that comprises a mixture of cells comprising adaptive immune cells and cells that are not adaptive immune cells. Methods are provided for amplifying by multiplex PCR and sequencing a first set of synthetic templates each comprising one TCR or IgV segment and one TCR of Ig J or C segment and a unique bar code. |
申请公布号 |
WO2015160439(A3) |
申请公布日期 |
2015.12.10 |
申请号 |
WO2015US18967 |
申请日期 |
2015.03.05 |
申请人 |
ADAPTIVE BIOTECHNOLOGIES CORPORATION |
发明人 |
ROBINS, HARLAN SAUL;CARLSON, CHRISTOPHER SCOTT;RIEDER, MARK J.;SHERWOOD, ANNA M.;EMERSON, RYAN O. |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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