发明名称 |
NANOPORE BASED MOLECULAR DETECTION AND SEQUENCING |
摘要 |
This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore. |
申请公布号 |
EP2807476(A4) |
申请公布日期 |
2015.12.09 |
申请号 |
EP20130738391 |
申请日期 |
2013.01.18 |
申请人 |
GENIA TECHNOLOGIES, INC. |
发明人 |
DAVIS, RANDALL;CHEN, ROGER |
分类号 |
G01N27/26;C12Q1/68;G01N27/414;G01N33/487 |
主分类号 |
G01N27/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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