METHOD FOR CULTURING NEURAL CREST STEM CELLS, AND USE THEREOF

摘要

Provided are a method for culturing peripheral nerve-derived neural crest stem cells including: culturing the peripheral nerve fragments embedded into the hydrogel; and breaking down only the hydrogel to collect neural crest stem cells that have migrated and proliferated in the hydrogel from the peripheral nerve fragments; and amplified and expanded collected neural crest stem cells in monolayer culture conditions. Provided also are a method of differentiating the neural crest stem cells; and a method of manufacturing cell therapeutic agent for the treatment of a neurologic disorder, each including the neural crest stem cells. Neural crest stem cells cultured according to the method have excellent in vitro proliferation capacity, and have the multipotency of being able to be differentiated into cells that constitute the peripheral nervous system and the central nervous system. Accordingly, the neural crest stem cells are suitable for use in the production of new drugs, such as the cell therapeutic agent and the tissue-engineering therapeutic agent, and also tools for cytological, molecular biological fundamental research and the new drug development research.