正调控花色苷合成的MYB转录因子的cDNA序列

摘要

本发明首次从红花香雪兰花瓣中克隆得到两条正调控花色苷合成的MYB转录因子(FhPAP1L1和FhPAP1L2)的cDNA序列，并推测得到了其编码的氨基序列。在拟南芥和烟草中过量表达FhPAP1L1和FhPAP1L2可以明显地增强植物组织的着色，从而确定其参与正调控植物花色苷合成的功能。

主权项

正调控香雪兰花朵花色苷合成的MYB转录因子的cDNA序列，其特征是：其中1：FhPAP1L1基因的全长核苷酸序列（SEQ ID NO. 1）ATCCAATATTCCTCCCCCTAATGTCTTATCTTGCATGATTCACAATTATGAAACATCAGTACTGCTCCGAGCTCCGACCTTCGGGCGTTCAGAATCGGAAAGGGGCATGGAACAAAGATGAAGATGAGCTGCTGAGGAAGTGCATTGAAAAGTATGGAATAGGGAAGTGGAGTACAGTTCCCATGAGGGCAGGACTAAGAAGGTGTCGCAAGAGCTGTCGTCTTCGCTGGCTGAACTATCTATCCCCTGACATCAACCGAGGAACCTTTAACGACGACGAAGACGACCTCATCCTTCGGCTTCACAAGCTCTTGGGCAATAGGTGGTCCCTAATTGCAGGCAGAATCCCCGGCAGGACGGCGAACGACATCAAGAACTACTGGAACTCTCACTTGGGCAAGAAAGCAGACACCGAGAGAAGGGTACCAGAGAAGACTGACAAGGCTGAGATTGTAAGGCCACAACCTCAAAGACCGACTTCGACGTGGATTTGGCCGAAGATTAATATTAATCCTCATCAAGAAACTGATCAATTAGGAACATCTGCCGTGGAGCCAAAACTGCCAACAGTCTTAGAGGAGGAAGAAGCATGGCTGAATGCTTTGATCACCGATGATTACAATGAGAATGTGGATGCCGCCTTTTCGGACTTCCATGGCTACGAGATAGGGGGAGGAATATTGGATGACACGCCTTTTTTCGAAGGAGTTACAGGATGGGAGGAACTATACCAGAGAACTGAAAATTAAGTTTAATTTTTCATTGTTCTAACAATACTGATATCTATATATATATTTTTTTTTTCCTTCGAAGATCAAAGATAGAAAGAAATGAGACCAGTTCCCCATTGTTGAAAAATAAAGGAATAAGGTTTATGTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGATGTCGACTCGAGTC；其中2：由FhPAP1L1基因核苷酸序列推测得到的蛋白质序列（SEQ ID NO. 2）MKHQYCSELRPSGVQNRKGAWNKDEDELLRKCIEKYGIGKWSTVPMRAGLRRCRKSCRLRWLNYLSPDINRGTFNDDEDDLILRLHKLLGNRWSLIAGRIPGRTANDIKNYWNSHLGKKADTERRVPEKTDKAEIVRPQPQRPTSTWIWPKININPHQETDQLGTSAVEPKLPTVLEEEEAWLNALITDDYNENVDAAFSDFHGYEIGGGILDDTPFFEGVTGWEELYQRTEN；其中3：FhPAP1L2基因的全长核苷酸序列（SEQ ID NO. 3）AATGGATCTCAAAGCTTCAGCCCTTCGAAAAGGCGCATGGAGCAAAGAGGAAGACGACCTGCTAAGGAATTGTATCGATAAGTATGGAGAAGGGAAGTGGAGTTCCGTACCCGAACGTGCAGGGCTTAAAAGGTGTCGAAAGAGCTGTCGACTTAGATGGCTGAACTATCTTTCGCCGAAGATCAACCGAGGCAAGTTTTCGGATGACGAGATCGACCTCCTTATCAGGCTTCACAAGCTTCTAGGGAACAGGTGGTCGCTGATCGCGGGCAGAATCCCCGGTAGGACAGCGAACGACATCAAGAATTATTGGAACTCTCACCTGAGCAAGAGAGTGGAAGCAACAAAATGTAGGAGCAAAACTATAGATGCTCAAGTCGCGAGGCCAAATCCTGAGAGAGATTCCGCCAACTGGAGTTGGCCAACAAATATAGGAACAAATTATGGGAACAACTTGGAAGCAGAAGAAACCGAAATGCCGAAACTACGAACAAGTCAAGAGAATCAGGGAGAACGGTCAAATGATTTGATCATGGAGAATGGTTGGGAAGAGTTGATGCTGCATACTTCTGACGTGAGCTTACAGAATTTTCAGATAGACTTCAAGACACCGGAAATGGAGGAACTAACTGAGATGGAGGAACTAACTGAGGAACCAATCTCTCTGGGAGATGAAGGGAATGATTGGACTGCCATGGAAAACTATTTTTTTGATGAGCTCATTAGTTAAGAAAAAAATATATACTAAAATGCGG；其中4：由FhPAP1L2基因核苷酸序列推测得到的蛋白质序列（SEQ ID NO.4）MDLKASALRKGAWSKEEDDLLRNCIDKYGEGKWSSVPERAGLKRCRKSCRLRWLNYLSPKINRGKFSDDEIDLLIRLHKLLGNRWSLIAGRIPGRTANDIKNYWNSHLSKRVEATKCRSKTIDAQVARPNPERDSANWSWPTNIGTNYGNNLEAEETEMPKLRTSQENQGERSNDLIMENGWEELMLHTSDVSLQNFQIDFKTPEMEELTEMEELTEEPISLGDEGNDWTAMENYFFDELIS。