Highly simplified lateral flow-based nucleic acid sample preparation and passive fluid flow control

摘要

Highly simplified lateral flow chromatographic nucleic acid sample preparation methods, devices, and integrated systems are provided for the efficient concentration of trace samples and the removal of nucleic acid amplification inhibitors. Methods for capturing and reducing inhibitors of nucleic acid amplification reactions, such as humic acid, using polyvinylpyrrolidone treated elements of the lateral flow device are also provided. Further provided are passive fluid control methods and systems for use in lateral flow assays.

主权项

1. A device for processing a sample comprising a material which defines a flow path and which comprises the following elements:
a sample receiving zone for receiving an aliquot of a fluid sample which comprises targets comprising nucleic acids; a capture zone in capillary flow contact with said sample receiving zone for binding and/or purifying the targets; a capillary flow path comprising said material and connecting said sample receiving zone and said capture zone; and one or more assay zones, each assay zone connected to said capillary flow path via two solution flow paths comprising said material, each said solution flow path forming a junction with said capillary flow path downstream of said sample receiving zone; each said solution flow path configured to deliver a corresponding solution to said capillary flow path or said capture zone at a desired time or sequence after the aliquot and each solution are all added to the device at the time of assay initiation; wherein for each assay zone said two junctions are on opposite sides of said capillary flow path; and wherein the material comprises an absorbent material capable of supporting fluid wicking and the passive control of at least one fluid flow therein.