Diagnostic method

摘要

The present invention relates to a multiplex in vitro nucleic acid amplification method for identifying a species of the Mycobacterium tuberculosis complex present in a sample. The method includes detecting the presence or absence of a plurality of nucleic acid molecule targets in the sample in one reaction, wherein at least one of the nucleic acid molecule targets is present in the genome of one or more, but not all, of the species of the Mycobacterium tuberculosis complex. The invention also includes kits containing primers or probes for conducting this method, nucleic acids useful for performing this method and diagnostic techniques using these nucleic acids.

主权项

1. A multiplex in vitro nucleic acid amplification method for identifying Mycobacterium canettii present in a sample, wherein the method comprises:
(i) performing a multiplex in vitro nucleic acid amplification using multiple sets of primers that are suitable for amplifying a plurality of nucleic acid molecule targets in the sample in one reaction, and (ii) detecting the presence or absence of a plurality of nucleic acid molecule targets in the sample in one reaction, wherein the plurality of nucleic acid molecule targets, in combination, are unique in their presence or absence to Mycobacterium canettii, and wherein primers or probes comprise a sequence of at least 16 contiguous nucleotides of SEQ ID NO:78, or the sequence complementary thereto, said primers or probes are specific for a nucleic acid sequence that is present in M. canettii, but is not present in M. tuberculosis and comprises a region of RDcanetti1, SEQ ID NO: 78, and are used in the amplification and/or detection step.