发明名称 |
Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
摘要 |
The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. |
申请公布号 |
US9206477(B2) |
申请公布日期 |
2015.12.08 |
申请号 |
US201414513906 |
申请日期 |
2014.10.14 |
申请人 |
Cornell Research Foundation, Inc. |
发明人 |
Barany Francis;Barany George;Hammer Robert P.;Kempe Maria;Blok Herman;Zirvi Monib |
分类号 |
C07H21/04;C12N15/11;C12Q1/68;B01J19/00;B82Y30/00;C40B40/06;C40B60/14 |
主分类号 |
C07H21/04 |
代理机构 |
LeClairRyan, a Professional Corporation |
代理人 |
LeClairRyan, a Professional Corporation |
主权项 |
1. A group of beads, wherein each bead of the group comprises one type of capture oligonucleotide from a collection of capture oligonucleotides, wherein each type of capture oligonucleotide is 20-24 nucleotides in length, and wherein each type of capture oligonucleotide comprises a nucleotide sequence that differs from the nucleotide sequence of other types of capture oligonucleotides of the collection by at least 25% when aligned, and wherein each capture oligonucleotide in the collection hybridizes to a nucleic acid molecule comprising a complementary nucleotide sequence under uniform hybridization conditions, wherein hybridized capture oligonucleotides comprise a melting temperature (Tm) that is >55° C. when calculated using the formula Tm=4(G+C)+2(A+T) ° C. |
地址 |
Ithaca NY US |