Micro-incubation systems for microfluidic cell culture and methods

摘要

A micro-incubator manifold for improved microfluidic configurations and systems and methods of manufacture and operation for a manifold and automated microfluidic systems.

主权项

1. A microfluidic cell culture system comprising:
at least one culture unit configured on a culture plate comprising:
a microfluidic culture chamber;a perfusion barrier separating said microfluidic culture chamber from one or more medium flow channels;a plurality of independent inlet channels in fluid communication with said one or more medium flow channels;andan outlet reservoir in fluid communication with said microfluidic culture chamber; and a pneumatic manifold; wherein the culture plate is positively sealed to said pneumatic manifold, allowing pressure driven control of the plurality of independent inlet channels so that solutions can be quickly changed over the cells or can be multiplexed or combined in the microfluidic culture chamber; wherein said pneumatic manifold further comprises a heat exchange module for providing a circulating heat controlled gas in a contained gas volume over said microfluidic culture chamber.