发明名称 |
REAGENTS AND METHODS FOR IMPROVING REPRODUCIBILITY AND REDUCING MISPRIMING IN PCR AMPLIFICATION |
摘要 |
<p>An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.</p> |
申请公布号 |
CA2584576(C) |
申请公布日期 |
2015.12.08 |
申请号 |
CA20052584576 |
申请日期 |
2005.10.17 |
申请人 |
BRANDEIS UNIVERSITY |
发明人 |
WANGH, LAWRENCE J.;RICE, JOHN;SANCHEZ, J. AQUILES;PIERCE, KENNETH;SALK, JESSE;REIS, ARTHUR;HARTSHORN, CRISTINA |
分类号 |
C12Q1/68;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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