| 摘要 |
PROBLEM TO BE SOLVED: To provide a microscope device capable of suppressing reduction in condensing intensity of irradiation light and spreading of the condensing shape of the irradiation light in a biological sample and to provide an image acquisition method.SOLUTION: A microscope device 1A includes a biological sample table 11 which supports a biological sample B, an objective lens 12 which faces the biological sample table 11, a laser light source 31 which irradiates the biological sample B with light through the objective lens 12, a shape measurement unit 20 which acquires the surface shape of the biological sample B, a control unit 40 which creates aberration correction hologram data for correcting an aberration caused by the surface shape of the biological sample B on the basis of information acquired in the shape measurement unit 20, a first spatial light modulator 33 to which a hologram based on the aberration correction hologram data is presented and which modulates the light with which the biological sample B is irradiated from the laser light source 31, and a detector 37 which detects the intensity of light L2 to be detected generated in the biological sample B. |
