COAGULATION FACTOR IX COMPOSITIONS AND METHODS OF MAKING AND USING SAME

摘要

The present invention relates to compositions comprising factor IX coagulation factors linked to extended recombinant polypeptide (XTEN), isolated nucleic acids encoding the compositions and vectors and host cells containing the same, and methods of making and using such compositions in treatment of coagulation factor-related diseases, disorders, and conditions.

主权项

1. A factor IX fusion protein comprising a factor IX polypeptide and an extended recombinant polypeptide (XTEN), said XTEN comprising an amino acid sequence having at least about 90% sequence identity to a sequence selected from SEQ ID NOs: 35, 36, 37, 38, 43, 44, 45, and 46, wherein the XTEN is characterized in that:
a. the sum of glycine (G), alanine (A), serine (S), threonine (T), glutamate (E) and proline (P) residues constitutes more than about 80% of the total amino acid residues of the XTEN; and b. the XTEN is non-repetitive such that (i) the XTEN contains no three contiguous amino acids that are identical unless the amino acids are serine, or (ii) at least 80% of the XTEN comprises non-overlapping sequence motifs, each of the sequence motifs comprising about 9 to about 14 amino acid residues, wherein any two contiguous amino acid residues do not occur more than twice in each of the sequence motifs.