SULFATED DEPOLYMERIZED DERIVATIVES OF EXOPOLYSACCHARIDES (EPS) FROM MESOPHILIC MARINE BACTERIA, METHOD FOR PREPARING SAME, AND USES THEROF IN TISSUE REGENERATION

摘要

The invention relates to certain low-molecular weight sulphated polysaccharide derivatives of marine native exopolysaccharides (EPSs) excreted by mesophilic marine bacteria from a deep hydrothermal environment, wherein said derivatives can be obtained by means of a method which comprises a step of free radical depolymerisation of said native EPSs followed by a step of sulphating the resulting depolymerised derivatives. The present invention further relates to the use of said low-molecular weight sulphated polysaccharide derivatives as a wound-healing agent, particularly for preparing pharmaceutical compositions suitable for treating or preventing diseases of the connective tissues and particularly skin and gum tissues. The figure demonstrates how polysaccharide derivative GY 785 DRS according to the invention can stimulate fibroblast proliferation in latticed or reconstructed connective tissues at a concentration of 10 μg (m) g/ml.

主权项

1. An adult mesenchymal stem cell proliferation medium comprising a low-molecular-weight sulfated polysaccharide derivative, wherein said low-molecular-weight sulfated polysaccharide derivative:
is a derivative of a marine native exopolysaccharide (EPS) excreted by a marine bacterium of the Alteromonas genus or Pseudoalteromonas genus or Vibrio genus; has a molecular weight of less than or equal to 100,000 g/mol, a polydispersity index of less than or equal to 2 and a degree of sulfate group substitution between 10% and 45% by weight inclusive, and is obtained by a process comprising:
a step consisting of free-radical depolymerisation of said native EPS so as to obtain a depolymerized derivative of low molecular weight, less than or equal to 100,000 g/mol, anda subsequent step consisting of sulfation of the depolymerized derivative, comprising adding to the depolymerised derivative at least one sulfation agent in an amount sufficient to obtain a sulfated polysaccharide derivative having a degree of sulfate group substitution of between 10% and 45% by weight relative to the total weight of the sulfated polysaccharide derivative.