MINIMAL BACTERIAL GENOME

摘要

The present invention relates, e.g., to a minimal set of protein-coding genes which provides the information required for replication of a free-living organism in a rich bacterial culture medium, wherein (1) the gene set does not comprise the 100 genes listed in Table 2; and/or wherein (2) the gene set comprises the 382 protein-coding genes listed in Table 3 and, optionally, one of more of: a set of three genes encoding ABC transporters for phosphate import (genes MG410, MG411 and MG412; or genes MG289, MG290 and MG291); the lipoprotein-encoding gene MG185 or MG260; and/or the glycerophosphoryl diester phosphodiesterase gene MG293 or MG385.

主权项

1. A non-naturally occurring free-living prokaryotic organism comprising a plurality of bacterial genes comprised on one or more nucleic acid molecules,
wherein the plurality of bacterial genes encode at least 351 proteins but not more than 450 proteins, and wherein:
(i) the at least 351 proteins are encoded by a minimal gene set and are required for growth and replication of a free-living bacterial organism under axenic conditions in a rich bacterial medium; and(ii) the at least 351 proteins perform at least the functions of the genes set forth in Table 3; and(iii) further comprising at least one expressible heterologous gene.