METHOD AND SYSTEM FOR DESIGNING POLYNUCLEOTIDE SEQUENCES AND POLYNUCLEOTIDE SEQUENCES OBTAINED THEREBY

摘要

Methods of designing a polynucleotide sequence for expressing a polypeptide-of-interest in a cell are provided. Also provided are artificial transcript sequences generated according to the present teachings. Further provided are methods of estimating the adaptiveness of a transcript sequence encoding a polypeptide-of-interest to a gene expression machinery in a cell.

主权项

1. A method of designing a polynucleotide sequence for expressing a polypeptide-of-interest (POI) in a host cell, the method comprising:
(a) identifying in a reference set of polynucleotide sequences of said host cell at least one string of genetic elements, wherein each of said at least one string is at least 4 nucleotides long and is capable of encoding a portion of said POI; and (b) embedding said at least one string in a transcript sequence encoding said POI.