Enzyme Hyaluronan-Lyase, Method of Production Thereof, Use Thereof and Method of Preparation of Low-Molecular Hyaluronan

摘要

The invention relates to an enzyme which is able to degrade hyaluronic acid and which is produced by fungi of the genus Fistulina (especially Fistulina hepatica). The degradation proceeds by lyase mechanism in which double bonds between C4 and C5 of glucuronic acid are formed. The invention also includes the process of preparation and purification of the enzyme and a possible practical use thereof for the preparation of low-molecular hyaluronan or of cosmetic or pharmaceutical devices. Further, the invention relates to the method of preparation of low-molecular hyaluronan.

主权项

1. A method of preparation of hyaluronan-lyase, characterized by that it is obtained by submerged cultivation of a fungus belonging to the genus Fistulina at the temperature of 20 to 30° C. for 5 to 11 days.