| 发明名称 |
PARTIAL PURIFICATION METHOD FOR HYDROPHILIC RECOMBINANT PROTEIN |
| 摘要 |
The present invention relates to a method for partially purifying a hydrophilic recombinant protein from a host expressing a hydrophilic recombinant protein. The method includes: a solution acquisition step in which a dissolving solvent is added to the host expressing a hydrophilic recombinant protein so as to dissolve the host cell and an insoluble substance is separated therefrom so as to obtain a solution. The hydrophilic recombinant protein has a hydropathy index of 0 or less, and the dissolving solvent is an aprotic polar solvent. Preferably, the solution is subjected to solvent substitution using an aqueous solvent. |
| 申请公布号 |
US2015344542(A1) |
申请公布日期 |
2015.12.03 |
| 申请号 |
US201314655027 |
申请日期 |
2013.12.18 |
| 申请人 |
SPIBER INC. |
发明人 |
OSAWA Toshiaki;MORITA Keisuke |
| 分类号 |
C07K14/78;C07K14/435;C07K1/14 |
主分类号 |
C07K14/78 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for partially purifying a hydrophilic recombinant protein from a host expressing a hydrophilic recombinant protein,
the method comprising a solution acquisition step in which a dissolving solvent is added to the host expressing a hydrophilic recombinant protein so as to dissolve the host cell and an insoluble substance is separated therefrom so as to obtain a solution, wherein the hydrophilic recombinant protein has a hydropathy index of 0 or less, and the dissolving solvent is an aprotic polar solvent. |
| 地址 |
Tsuruoka-shi, Yamagata JP |
