METHODS OF SYNTHESIS OF OLIGONUCLEOTIDE TAGGED COMBINATORIAL LIBRARIES AND USES THEREOF

摘要

Provided herein are methods of synthesis of a compound which includes a functional moiety operatively linked to a tagging oligonucleotide, libraries thereof and methods of using the compound and libraries thereof to identify compounds which bind to a target.

主权项

1. A method of synthesizing a library of compounds wherein each compound comprises a functional moiety comprising two or more building blocks operatively linked to a tagging oligonucleotide:
a. splitting a functional moiety operatively linked with an oligonucleotide into multiple fractions; b. reacting the functional moiety with a unique tagging oligonucleotide; c. optionally, hybridizing the functional moiety with a complementary oligonucleotide; d. non-covalently immobilizing the compound of step b or step c on a substance; e. reacting the functional moiety with a unique building block; f. eluting the functional moiety from the substance; g. combining the multiple fractions; and h. optionally, repeating steps a-g, j times wherein j is an integer greater than or equal to 1.