RECOMBINANT NON-PATHOGENIC MAREK'S DISEASE VIRUS CONSTRUCTS ENCODING INFECTIOUS LARYNGOTRACHEITIS VIRUS AND NEWCASTLE DISEASE VIRUS ANTIGENS

摘要

The present invention discloses novel recombinant multivalent non-pathogenic Marek's Disease virus constructs that encode and express both Infectious Laryngotracheitis Virus and Newcastle Disease virus protein antigens, and methods of their use in poultry vaccines.

主权项

1. A recombinant nonpathogenic Marek's Disease Virus (rMDVnp) comprising a first nucleic acid inserted in a first nonessential site in the rMDVnp genome and a second nucleic acid inserted in a second nonessential site in the rMDVnp genome;
wherein the first nucleic acid comprises both a nucleotide sequence that encodes an Infectious Laryngotracheitis Virus (ILTV) gD protein and a nucleotide sequence that encodes an Infectious Laryngotracheitis Virus (ILTV) gI protein; wherein the second nucleic acid comprises a nucleotide sequence that encodes a Newcastle Disease Virus fusion protein (NDV F); and wherein the first nonessential site and the second nonessential site are either the same or only the first nonessential site is the US2 site.