Pairing Code Directed Assembly

摘要

Provided herein is a method comprising: producing a first complex by annealing: a first nucleic acid comprising, in order, a first unique sequence, a first central sequence and a second unique sequence; and a second nucleic acid comprising, in order, said second unique sequence, a second central sequence and a third unique sequence; wherein the first, second and third unique sequences do not hybridize with each other; subjecting said first complex to multiple rounds of primer extension to extend the first and second nucleic acids using each other as a template, thereby producing a first product molecule that contains, in order, the first unique sequence, the first central sequence, the second unique sequence, the second central sequence and the third unique sequence; and circularizing said first product molecule by intramolecularly ligating the ends of said product molecule together. Kits and compositions relating to the method are also provided.

主权项

1. A method comprising:
(a) producing a first complex by annealing:
(i) a first nucleic acid comprising, in order, a first unique sequence, a first central sequence and a second unique sequence; and(ii) a second nucleic acid comprising, in order, said second unique sequence, a second central sequence and a third unique sequence;wherein the first, second and third unique sequences do not hybridize with each other; (b) subjecting said first complex to multiple rounds of primer extension to extend the first and second nucleic acids using each other as a template, thereby producing a first product molecule that contains, in order, the first unique sequence, the first central sequence, the second unique sequence, the second central sequence and the third unique sequence; and (c) circularizing said first product molecule by intramolecularly ligating the ends of said product molecule together.