METHOD FOR SEPARATING TARGET DNA USING INACTIVATED TARGET-SPECIFIC NUCLEASE

摘要

The present invention relates to a genotyping method using target-specific nuclease and, specifically, to a method for cancer diagnosis or genotyping by removing wild type DNA or particular genotype DNA using target-specific nuclease or a variant thereof to amplify or concentrate only a small amount of DNA which has a difference in variation, such as mutation, or genotype, and to a method for separating target DNA using target-specific nuclease or a variant thereof. Such methods are novel paradigm methods contrary to existing simple target-specific nuclease for post-PCR recognition of normal genotype and carcinogenic genotype, and can be favorably used in the early diagnosis of cancer or analysis of similar genotypes.