摘要 |
One aspect as reported herein is using a method comprising the step of immunizing an experimental animal, three times with primary cynomolgus PBLs, whereby the PBLs are optionally enriched for T cells without using primary human PBLs as immunogen and without using a denaturing agent for producing a human cynomolgus cross-reactive antibody specifically binding to human CD3 epsilon of SEQ ID NO: 02 and specifically binding to a polypeptide of SEQ ID NO: 01, wherein the human cynomolgus cross-reactive antibody specifically binds to human and cynomolgus T cells, activates human T cells and does not bind to the same epitope as the antibody OKT3, the antibody UCHT1 and/or antibody the SP34. |
申请人 |
F. HOFFMANN-LA ROCHE AG;HOFFMANN-LA ROCHE INC. |
发明人 |
TIEFENTHALER, GEORG;MOESSNER, EKKEHARD;LIFKE, VALERIA;PLATZER, JOSEF;OFFNER, SONJA;JAEGER, CHRISTIANE;RITTER, MIRKO |