METHOD FOR IN VITRO ESTIMATION OF TUMORIGENESIS, METASTASIS, OR LIFE EXPECTANCY AND ARTIFICIAL NUCLEOTIDE USED

摘要

A method of detection of occurrence of cancers comprising:
Extracting tissue or plasma free DNA from cancer and non-cancer patients;
Detecting and calculating proportion of methylated or demethylated GFRa1 CpG islands; setting a cutoff value using the proportion of methylated or demethylated GFRa1;
Extracting tissue or plasma free DNA samples from testing subjects in vitro, detecting and calculating proportion of methylated or demethylated GFRa1 CpG islands in samples;
Comparing proportion of methylated or demethylated GFRa1 from step c) with the cutoff value from step b);
If proportion of methylated GFRa1 from step c) is less than or equal to cutoff value from step b), the occurrence of cancer is considered, otherwise, it is not; or, if the proportion of demethylated GFRa1 from step c) is higher than or equal to cutoff value from step b), the occurrence of cancer is considered, otherwise it is not considered.