发明名称 |
METHOD AND DEVICE FOR OPTIMIZING A NUCLEOTIDE SEQUENCE FOR THE PURPOSE OF EXPRESSION OF A PROTEIN |
摘要 |
Computer optimization of a nucleotide sequence for a protein comprises generating a test sequence; establishing positions corresponding to codons to be optimized; generating further test sequences by replacing one or more of these codons with another codon for the same amino acid; evaluating the test sequences with a quality function to determine the optimum sequence; establishing codons of the optimum sequence as success codons; and iterating the above steps without changing the success codons. Computer optimization of a nucleotide sequence for expression of a protein comprising N amino acids comprises generating a test sequence of n codons corresponding to n contiguous amino acids of the protein sequence, where n is =N; establishing m positions corresponding to codons to be optimized, where m is = n and less than N; generating one or more further test sequences by replacing one or more of the m codons with another codon encoding the same amino acid; evaluating the test sequences with a quality function to determine the optimum sequence; establishing p codons of the optimum sequence as success codons, where p is =m; and iterating the above steps without changing the success codons. Independent claims are also included for: (1) computer apparatus for optimization of a nucleotide sequence for expression of a protein, comprising means for performing the above steps; (2) computer program for performing the above method; (3) data carrier on which the above program is stored in computer-readable form; (4) nucleic acid (I) comprising a protein-encoding nucleotide sequence produced by synthesizing the sequence optimized as above; (5) nucleic acid (II) comprising one of four defined nucleotide sequences; (6) vector containing (I) or (II); (7) cells containing the vector or (I) or (II); (8) organism comprising at least one cell as above. |
申请公布号 |
EP1584058(B1) |
申请公布日期 |
2015.12.02 |
申请号 |
EP20030785937 |
申请日期 |
2003.12.23 |
申请人 |
GENEART AG |
发明人 |
RAAB, DAVID;GRAF, MARCUS;NOTKA, FRANK;WAGNER, RALF |
分类号 |
G06F19/18;C07H21/00;C12M1/36;C12N15/11;C12N15/12;C12N15/19;C12N15/24;C12N15/27;C12N15/29;C12N15/31;C12N15/52;C12P21/00;G06F19/22 |
主分类号 |
G06F19/18 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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