主权项 |
1. A method for identifying bacterial vaginosis in a human female subject, the method comprising:
(a) obtaining a vaginal swab sample from the subject; (b) measuring levels of Atopobium vaginae, Megasphaera genus and one or more Lactobacilli species selected from the group consisting of Lactobacillus acidophilus, Lactobacillus crispatus, and Lactobacillus jensenii in the sample, wherein the levels of Atopobium vaginae, Megasphaera genus and of one or more Lactobacilli species are detected with one or more oligonucleotides capable of specifically hybridizing to a target nucleic acid sequence from Atopobium vaginae, Megasphaera genus, Lactobacillus acidophilus, Lactobacillus crispatus, and/or Lactobacillus jensenii, wherein levels of Gardnerella vaginalis and Mobiluncus sp are not measured; wherein the levels are determined with one or more oligonucleotide primers selected from SEQ ID NOs: 1, 2, 4, 5, 11, 12, 14 and 15, and complements thereof; (c) calculating a diagnostic score as the ratio of a logarithmic function of the levels of the one or more Lactobacilli species and a logarithmic function of the levels of the Atopobium vaginae and Megasphaera genus; and (d) identifying the subject as having bacterial vaginosis with the diagnostic score lower than a reference score. |