发明名称 |
Methods and compositions for targeted single-stranded cleavage and targeted integration |
摘要 |
Disclosed herein are methods and compositions for generating a single-stranded break in a target sequence, which facilitates targeted integration of one or more exogenous sequences. |
申请公布号 |
US9200266(B2) |
申请公布日期 |
2015.12.01 |
申请号 |
US201414199753 |
申请日期 |
2014.03.06 |
申请人 |
Sangamo BioSciences, Inc. |
发明人 |
Wang Jianbin |
分类号 |
C12N15/01;C12N15/00;C12N15/87;C12P19/34;A61K38/43;C07H21/04;C07K1/00;C12N9/22;C12N15/90 |
主分类号 |
C12N15/01 |
代理机构 |
Pasternak Patent Law |
代理人 |
Pasternak Patent Law ;Abrahamson Susan |
主权项 |
1. An artificial nuclease comprising
(i) first and second cleavage domains from an endonuclease, wherein the first cleavage domain is catalytically inactive and the second cleavage domain is catalytically active; and (ii) a DNA-binding molecule that is heterologous to the first and second cleavage domains, and further wherein the DNA-binding molecule of the nuclease binds to a target sequence in a double-stranded genome and the nuclease induces a site-specific single-stranded break at or near the target sequence in the double-stranded genome such that the double-stranded genome is modified. |
地址 |
Richmond CA US |