Differentiation of adipose stromal cells into osteoblasts and treating bones

摘要

The invention provides methods and compositions for differentiating stromal cells from adipose tissue into cells having osteoblastic properties, and methods for improving a subject's bone structure. The methods comprise culturing stromal cells from adipose tissue in β-glycerophosphate and ascorbic acid and/or ascorbate-2-phosphate for a time sufficient to allow differentiation of said cells into osteoblasts. Such methods and compositions are useful in the production of osteoblasts for autologous transplantation into bone at a surgical site or injury. The compositions comprise adipose stromal cells, a medium capable of supporting the growth of fibroblasts and amounts of β-glycerophosphate and ascorbic acid and/or ascorbic-2 phosphate sufficient to induce the differentiation of said stromal cells into osteoblasts.;The invention further provides methods of identifying compounds that affect osteoblast differentiation. Such compounds are useful in the study of bone development and in the treatment of bone disorders, including bone fractures and osteoporosis.

主权项

1. A method of treating a bone wound, bone fracture or bone disorder in a subject in need of increased bone formation, comprising:
a) culturing adipose stromal cells (ASCs) in a cell culture medium capable of supporting the growth of fibroblasts, the medium comprising an effective amount of (β-glycerophosphate and ascorbic acid and/or ascorbic-2 phosphate to induce differentiation of the ASCs, wherein the differentiated ASCs have positive von Kossa staining, secrete osteocalcin and/or have an elevated secreted alkaline phosphatase activity, and b) introducing the differentiated adipose stromal cells into a site of a bone wound, bone fracture or bone disorder of the subject in need of increased bone formation, thereby treating the wound, fracture or disorder.