Compositions for inducing differentiation into retinal cells from retinal progenitor cells or inducing proliferation of retinal cells comprising Wnt signaling pathway activators

摘要

Disclosed is a composition for inducing the proliferation of retinal cells or the differentiation of retinal progenitor cells into retinal cells. The composition, similar to in vivo conditions for development during embryogenesis, induces stem cells to differentiate into a multitude of photoreceptor cells at high yield within a short period of time, without an additional gene transfer. In addition, the differentiated photoreceptor cells are useful in cellular therapy because they, when transplanted into degenerated or injured retinas, can be engrafted and fused within the retinas to prevent or cure retinal degeneration.

主权项

1. A method for producing retinal cells comprising:
a) culturing the human ESCs or IPSCs as floating aggregates in a medium comprising IGF-1 DKK-1, and NOGGIN to differentiate the human embryonic stem cells into eye field precursor cells; b) culturing the eye field precursor cell floating aggregates in a medium comprising IGF-1 DKK-1, NOGGIN, and FGF-2 to differentiate the eye field precursor cells into retinal progenitor cells; c) inducing differentiation of the retinal progenitor cells to retinal cells by culturing the retinal progenitor cells in a medium comprising a differentiation factor selected from the group consisting of: a Wnt signaling pathway activator; wherein the medium does not comprise exogenous retinoic acid and exogenous sonic hedgehog.