Flagellin fusion proteins

摘要

Methods of making a protein that stimulates a protective immune response in a subject include separating a portion of a protein from a naturally occurring influenza viral hemagglutinin to form a protein portion.

主权项

1. A method of making an immunogenic fusion protein, comprising the steps of:
a) transforming a nucleic acid encoding a fusion protein into a prokaryotic host cell, the fusion protein including,
i) at least a portion of a flagellin that initiates an intracellular signal transduction pathway for a Toll-like Receptor, andii) a portion of an influenza viral hemagglutinin, wherein the portion of the influenza viral hemagglutinin lacks
a transmembrane domain; anda cytoplasmic domain, and includes, at least a portion of a globular head; and at least a portion of at least one secondary structure having at least one β-sheet at a bottom of the globular head that causes the globular head to essentially retain its tertiary structure; and b) culturing the prokaryotic host cell to thereby make the immunogenic fusion protein.