Dried oligonucleotide composition and method of producing the same

摘要

The present invention relates to a dried oligonucleotide composition and a method for producing the same. More specifically, it relates to a dried oligonucleotide composition produced by the steps comprising adding a substance for preventing the oligonucleotide from being separated and lost, which is adhesive to a storage container containing the oligonucleotide composition, in order to prevent the oligonucleotide from being separated and lost during manufacturing and distributing the dried oligonucleotide composition, optionally adding a non-reactive dye substance, and drying the resulting solution. The dried oligonucleotide composition of the present invention can be prevented from being separated and lost during manufacturing step, or transporting step after packaging, and the presence or absence of the oligonucleotide in the storage container can be easily confirmed with naked eyes. Accordingly, unnecessary labor waste and time waste caused by the separation of the oligonucleotide upon experiment can be overcome.

主权项

1. A composition for preventing the loss of oligonucleotide from a storage container consisting of a mixed solution consisting of an oligonucleotide, polyalcohol and water for preventing the oligonucleotide from being separated and lost, and optionally one or more non-reactive dye substance selected from the group consisting of bromophenol blue, xylene cyanole, bromocresol red and cresol red,
wherein said mixed solution contains said polyalcohol in an amount of 0.01 to 5 parts by weight based on 100 parts by weight of the oligonucleotide, and wherein said composition is adhesive to said storage container after said solution is aliquoted into and dried in said storage container to prevent the loss of dried oligonucleotide from said storage container, and wherein said polyalcohol is chemically non-reactive with the oligonucleotide, and does not give substantive influence on PCR reaction.