| 发明名称 | Anti-cocaine compositions and treatment | ||
| 摘要 | Embodiments of the invention disclosed herein generally relate to anti-cocaine therapeutics. Specifically, some embodiments of the invention relate to highly efficient, thermostable, and long-lasting cocaine esterase (CocE) mutants that can protect against the toxic and reinforcing effects of cocaine in subjects. Provided herein are mutant CocE polypeptides displaying thermostable esterase activity. Also provided are methods of treating cocaine-induced conditions in a subject in need via administration of mutant CocE as well as methods for high-throughput screening of candidate esterase polypeptides. | ||
| 申请公布号 | US9200265(B2) | 申请公布日期 | 2015.12.01 |
| 申请号 | US201213676324 | 申请日期 | 2012.11.14 |
| 申请人 | The Trustees of Columbia University in the City of New York;The Regents of the University of Michigan;University of Kentucky Research Foundation | 发明人 | Landry Donald;Zhan Chang-Guo;Woods James H.;Sunahara Roger;Narasimhan Diwahar L.;MacDonald Joanne;Yang Victor;Ko Mei-Chuan Holden;Deng Shi-Xian;Tesmer John J.;Lee Tien-Yi;Kwon Young Min;Gao Daquan |
| 分类号 | A61K38/46;A61K38/43;C12N9/18;C12N9/16;C12N9/14;C12P21/06;C12P19/34;C07H21/04;C07K1/00;A61K38/00 | 主分类号 | A61K38/46 |
| 代理机构 | Dentons US LLP | 代理人 | Dentons US LLP |
| 主权项 | 1. An isolated mutant cocaine esterase (CocE) polypeptide comprising an amino acid sequence at least 90% identical to SEQ ID NO: 1, having at least one substitution, addition or deletion, wherein the at least one substitution, addition or deletion is selected from the group consisting of L163V; V225I; I218L; A310D; A149S; S159A; S265A; S56G; W220A; S140A; F189L; A193D; T254R; N42V; V262L; L508G; Y152H; V160A; T172R; Y532F; T74S; W285T; L146P; D533S; A194R; G173Q; C477T; K531A; R41I; L119A; K46A; F84Y; T172R-G173Q; L169K; F189A; N197K; R182K; F189K; V190K; Q191K; A194K; I175-G-G-A186; and T176-G-G-D185; andthe mutant CocE polypeptide has esterase activity. | ||
| 地址 | New York NY US | ||