Co-culture device assembly

摘要

An insert device and co-culture device assembly incorporating the insert device, which includes an insert chamber with two fluid impermeable side walls that extend from a microporous bottom to an open top to form an inner cavity. The first side wall is shaped to form a convex arc that follows between about half of a circumference of the well and less than an entire circumference of the well. The second side wall joins ends of the first side wall and protrudes inward towards a center of the convex arc. A flange extends outward from or beneath the top and is notched to form a gap adjacent to the second side wall, which forms an access port allowing access to the lower chamber with a micropipette tip when the insert device is inserted into a well of a single or multi-well plate.

主权项

1. A co-culture device assembly comprising an insertion device suspended above a bottom surface of a well to form an upper chamber for housing cells over a lower chamber, wherein the insertion device is characterized as:
a) an insert chamber comprising two fluid impermeable side walls extending from a microporous bottom to an open top to form an inner cavity, characterized in that first and second side walls are each shaped convex and are joined to one another at each end, wherein the first and second side walls are of a same height; and b) a flange extending outward from or beneath the top of the insert chamber and suspending the microporous bottom above the bottom surface of the well to form the upper and lower chambers, the flange notched to form a gap providing access to the lower chamber but not the top chamber, wherein a bottom surface of the flange comprises an upward extending recess that recesses into the flange for preventing or reducing liquid migration across the bottom surface of the flange.