| 主权项 |
1. A method of euthanizing one or more animals comprising the steps of:
placing the one or more animals into a holding cage, placing a lid onto the cage, placing the cage into a euthanizing chamber, and closing a chamber door wherein the euthanizing chamber is sealed; wherein the euthanizing chamber is connected to: a spray-able liquid anesthesia source and a valve to control a volume of liquid anesthesia that is sprayed, a compressed carbon dioxide gas source, at least one regulator to control a carbon dioxide gas flow rate, and at least one valve to control the duration of carbon dioxide flow into the euthanizing chamber; wherein the euthanizing chamber comprises: three inlets; at least one exhaust outlet connected to an exhaust vent or an activated charcoal filter; an anesthetic evaporation plate or evaporation chamber; and a venturi vacuum pump that is configured to spray the liquid anesthesia using the regulated compressed carbon dioxide gas to create a vacuum and propel the liquid anesthesia; wherein the euthanizing chamber houses one or more holding cages; spraying the liquid anesthesia into the euthanizing chamber using the venturi vacuum pump and onto the evaporation plate or into the evaporation chamber, followed by a first wait period to anesthetize the animals; after the first wait period is completed, opening the at least one valve to introduce regulated carbon dioxide gas from the compressed carbon dioxide gas source into the euthanizing chamber at a predetermined flow rate for a predetermined duration until the carbon dioxide gas displaces the liquid anesthesia and accounts for approximately 100% of an internal atmosphere inside the euthanizing chamber; ceasing introduction of the carbon dioxide gas into the euthanizing chamber for a second wait period to ensure irreversible euthanasia of the one or more animals; after the second wait period is completed, removing and disposing of carcasses of the one or more animals. |
