Microscopy method and system incorporating nanofeatures

摘要

A lensfree imaging and sensing device includes an image sensor comprising an array of pixels and a substantially optically transparent layer disposed above the image sensor. Nano-sized features that support surface plasmon waves are populated on the substantially optically transparent layer separating the image sensor from the nano-sized features. The nano-sized features may include apertures through a substantially optically opaque layer (e.g., metal layer) or they may include antennas. An illumination source is provided that is configured to illuminate a sample. At least one processor is operatively coupled to the image sensor. Changes to the detected transmission pattern at the image sensor are used to sense conditions at or near the surface containing the nano-sized features. Conditions may include binding events or other changes to the index of refraction occurring near the surface of the device.

主权项

1. A lensfree imaging and sensing device for imaging a temporary or permanently stationary object within a sample comprising:
an image sensor comprising an array of pixels; a substantially optically transparent layer disposed above the image sensor; a substantially optically opaque layer disposed above the substantially optically transparent layer, the substantially optically opaque layer comprising a plurality of apertures extending through the substantially optically opaque layer, wherein a plurality of separate apertures overlie a single pixel of the sensor array and wherein each of the plurality of separate apertures overlying a single pixel of the sensor array exhibits a resonant transmission predominantly at a single wavelength and polarization combination, (λ,p)n; an illumination source configured to illuminate the sample, wherein an optical path defined between the object and the image sensor is free of any optical lenses; and at least one processor operatively coupled to the image sensor.