Methods and compositions for amplifying a detectable signal

摘要

Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.

主权项

1. An amplification polymer for use in a diagnostic assay, comprising:
a polymer having a plurality of amine groups; a plurality of detectable labels conjugated to a portion of the amine groups; a plurality of amine capping groups conjugated to a portion of the plurality of amine groups not conjugated to the detectable labels; wherein the amplification polymer is neutrally charged and soluble in water.