Generation of engineered molecular weight standards

摘要

The invention provides methods of generating physical analog polypeptides, and polypeptides generated employing the methods of the invention, that have the same amino acid composition of a reference protein, but have an unrelated primary sequence. The invention further provides compositions and methods employing such proteins.

主权项

1. A molecular weight marker composition that comprises an engineered polypeptide that comprises an amino acid sequence having at least 80% identity to an amino acid sequence set forth in SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, or SEQ ID NO:8; wherein the protein characterized by the amino acid sequence retains the same charge and molecular weight as a reference protein set forth in SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, or SEQ ID NO:7.