Use of entrained neutrophils to treat metastatic and micrometastatic disease in at risk patients

摘要

The present invention relates generally to compositions and methods for treating cancer patients with a poor prognosis, and to therapeutic modalities for improving prognosis by combating metastasis and abrogating chemoresistance in cancer cells. In particular, the invention relates to the role of white blood cells, i.e. neutrophils and neutrophil-like cells, in preventing the spread of cancer from a primary tumor to secondary locations in the body. The invention provides methods for reducing or delaying the spread of metastatic cancer cells in a patient at risk for metastatic tumor development, at risk for metastatic relapse, i.e. prophylactic methods, and treating patients suffering from metastatic tumors.

主权项

1. A method, comprising,
a) providing,
i) a patient at risk for developing metastatic cancer, wherein said patient comprises a neutrophil, wherein said neutrophil is capable of being cytotoxic to a tumor cell; andii) a chemokine selected from the group consisting of Chemokine (C-C motif) ligand 2, Chemokine (C-C motif) ligand 3, Chemokine (C-C motif) ligand 5, chemokine (C-X-C motif) ligand 1, chemokine (C-X-C motif) ligand 12, chemokine (C-X-C motif) ligand 16, capable of increasing said neutrophil cytotoxicity; b) isolating said neutrophil from said patient; and c) contacting said neutrophil with said chemokine under conditions for increasing cytotoxic activity of said neutrophil.