Method for culturing photoautotrophic microorganisms for the production of biomass

摘要

The invention relates to a method for cultivating photoautotrophic micro-organisms, comprising the following steps: provision of a photosynthesis layer, which contains a photosynthesis medium and photoautotrophic micro-organisms suspended therein, in a closed accommodation space having a gas-diffusion-inhibiting outer boundary, wherein the photosynthesis layer has an average thickness of no more than 10 mm; exposure of the photosynthesis layer without eddying or feeding through a gas in the photosynthesis medium while limiting the diffusive gas exchange of the accommodation space by the environment, wherein the supply of micro-organisms in the photosynthesis medium with hydrogen carbonate ions is maintained by storing hydrogen carbonate ions in the photosynthesis medium and/or providing a photosynthesis substrate in the form of carbon dioxide and hydrogen carbonate from a cell-free carbon storage medium outside the photosynthesis layer; and conducting a gas formed during photosynthesis out of the accommodation space.

主权项

1. A method for the cultivation of photoautotrophic microorganisms for production of a biomass comprising:
Providing a closed space with an outer border (1) acting as a barrier for gas diffusion comprising at least one first compartment enclosing a photosynthesis layer (2) with a mean thickness of not more than 10 mm that contains a photosynthesis medium and photoautotrophic microorganisms suspended therein and at least one second compartment enclosing a cell-free carbon storage medium (4) that is separated from the first compartment by a porous hydrophilic membrane (3) and is transparent to light; Exposing the photosynthesis layer (2) to light without whirling a gas within the photosynthesis medium or piping a gas through the photosynthesis medium; Maintaining a bicarbonate concentration of at least 10 mM in the photosynthesis layer by diffusion of bicarbonate through the porous hydrophilic membrane from the cell-free carbon storage medium; and Convective channeling away of the gas volume formed by photosynthesis through an appliance for a gas outlet that restricts diffusive gaseous exchange, wherein the photoautotrophic microorganisms form a biomass.