发明名称 |
NANOPROBE-BASED GENETIC TESTING |
摘要 |
The present application relates to methods of detecting a mutation in a target nucleic acid molecule. Two phosphorodiamidate morpholino oligomer probes that differ by at least one base are each covalently coupled to a nano article and hybridised to a target sequence. The melting temperature of the complexes between each of the two probes and the target nucleic acid are measured and compared to determine whether the sample contains a nucleic acid with the mutation. Further, the present invention relates to kits comprising a first and second conjugate as described herein and to the use of such kits for the detection of mutations in a target nucleic acid molecule or for assigning a genotype to a target nucleic acid molecule. |
申请公布号 |
SG11201508345T(A) |
申请公布日期 |
2015.11.27 |
申请号 |
SGT11201508345 |
申请日期 |
2014.04.04 |
申请人 |
AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH |
发明人 |
ZU, YANBING;YING, JACKIE Y. |
分类号 |
C12Q1/68;B82Y15/00;C07H21/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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