| 摘要 |
Disclose a method of isolating chlorophylls and omega-3 rich oil from algae, comprising: a. dewatering substantially intact algal cells to make an algal biomass; b. adding a first ethanol fraction to the algal biomass in a ratio of about 1 part ethanol to about 1 part algal biomass by weight; c. separating a first substantially solid biomass fraction from a first substantially liquid fraction comprising proteins; d. combining the first substantially solid biomass fraction with a second ethanol fraction in a ratio of about 1 part ethanol to about 1 part solids by weight; e. separating a second substantially solid biomass fraction from a second substantially liquid fraction comprising polar lipids; f. combining the second substantially solid biomass fraction with a third ethanol solvent fraction in a ratio of about 1 part ethanol to about 1 part substantially solid biomass by weight; g. separating a third substantially solid biomass fraction from a third substantially liquid fraction comprising neutral lipids, including omega-3 fatty acids, carotenoids, and chlorophyll, wherein the third substantially solid biomass fraction comprises carbohydrates; and h. isolating at least one of carotenoids, chlorophyll, and omega-3 fatty acids from the third substantially liquid fraction. |
