Core Transcriptional Circuitry in Human Cells and Methods of Use Thereof

摘要

Disclosed herein are methods for identifying the core regulatory circuitry or cell identity program of a cell or tissue, and related methods of diagnoses, screening, and treatment involving the core regulatory circuitry and/or cell identity programs identified using the methods.

主权项

1. A method of identifying the core regulatory circuitry of a cell or tissue, comprising:
a) identifying a group of transcription factor encoding genes in a cell or tissue which are associated with a super-enhancer; b) determining which transcription factor encoding genes identified in a) comprise autoregulated transcription factor encoding genes, wherein a transcription factor encoding gene identified in a) comprises an autoregulated transcription factor encoding gene if the transcription factor encoded by the transcription factor encoding gene is predicted to bind to the super-enhancer associated with the transcription factor encoding gene; c) identifying the core regulatory circuitry of the cell or tissue, wherein the core regulatory circuitry of the cell or tissue comprises autoregulated transcription factor encoding genes identified in b) which form an interconnected autoregulatory loop, wherein the autoregulated transcription factor encoding genes identified in b) form an interconnected autoregulatory loop if each transcription factor encoded by an autoregulated transcription factor encoding gene identified in b) is predicted to bind to the super-enhancer associated with each of the other autoregulated transcription factor encoding genes identified in b).