发明名称 |
INTRAVITREAL INJECTION OF A CHIMERIC PHAGE ENDOLYSIN PLY187; PROTECTION FROM STAPHYLOCOCCUS AUREUS ENDOPHTHALMITIS |
摘要 |
The treatment of endophthalmitis is challenging due to the emergence of MDR bacteria. We evaluated the therapeutic potential of Ply187AN-KSH3b, a chimeric phage endolysin derived from the Ply187 prophage in a mouse model of Staphylococcus aureus endophthalmitis. The endolysin was injected intravitreally in C57BL/6 mouse eyes at 6h and 12h post S. aureus infection. The disease progression was monitored by ophthalmoscopic, electroretinography, histological, cell death and microbiological parameters. Expression of cytokines/chemokines and cellular infiltration was assessed. Intravitreal injection of chimeric Ply187AN-KSH3b (both at 6 and 12 h post infection) significantly improved the outcome of staphylococcal endophthalmitis, preserved retinal structural integrity, and maintained visual function. Phage lysin treatment significantly reduced the bacterial burden and the levels of inflammatory cytokines and neutrophil infiltration in the eyes. This is the first study demonstrating the therapeutic use of phage-based antimicrobials in ocular infections |
申请公布号 |
WO2015179390(A1) |
申请公布日期 |
2015.11.26 |
申请号 |
WO2015US31566 |
申请日期 |
2015.05.19 |
申请人 |
THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF AGRICULTURE;WAYNE STATE UNIVERSITY |
发明人 |
DONOVAN, DAVID M.;SINGH, PAWAN KUMAR;KUMAR, ASHOK |
分类号 |
A61K38/47;A61K9/08;A61K48/00;A61P27/02 |
主分类号 |
A61K38/47 |
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