发明名称 |
IDENTIFYING AND MEASURING RETICULOCYTES |
摘要 |
Methods and systems for identifying reticulocytes in a blood sample deposited on a substrate include: illuminating the sample with incident light at two different wavelengths, obtaining a two-dimensional image of the sample corresponding to a first one of the wavelengths, and obtaining a two-dimensional image of the sample corresponding to a second one of the wavelengths; analyzing the images to identify a set of representative red blood cells; determining an area of each of the red blood cells in the set; determining a color value of each of the red blood cells in the set; and, for each one of the red blood cells in the set, identifying the red blood cell as a reticulocyte if the area of the red blood cell exceeds an area cutoff value and the color value of the red blood cell is less than a color cutoff value. |
申请公布号 |
US2015339813(A1) |
申请公布日期 |
2015.11.26 |
申请号 |
US201514629058 |
申请日期 |
2015.02.23 |
申请人 |
Roche Diagnostics Hematology, Inc |
发明人 |
Zahniser Michael;Zahniser Russell |
分类号 |
G06T7/00;G06K9/52;G06K9/46;G01N15/14;H04N7/18 |
主分类号 |
G06T7/00 |
代理机构 |
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代理人 |
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主权项 |
1. A method for identifying reticulocytes in a blood sample deposited on a substrate, the method comprising:
illuminating the sample with incident light at two different wavelengths, obtaining a two-dimensional image of the sample corresponding to a first one of the wavelengths, and obtaining a two-dimensional image of the sample corresponding to a second one of the wavelengths; analyzing the images to identify a set of representative red blood cells; determining an area of each of the red blood cells in the set; determining a color value of each of the red blood cells in the set; and for each one of the red blood cells in the set, identifying the red blood cell as a reticulocyte if the area of the red blood cell exceeds an area cutoff value and the color value of the red blood cell is less than a color cutoff value, wherein the color value of each one of the red blood cells comprises a difference between optical density values of the red blood cell at the two illumination wavelengths. |
地址 |
Westborough MA US |