METHODS FOR CREATING BILAYERS FOR USE WITH NANOPORE SENSORS

摘要

The present disclosure provides biochips and methods for making biochips. A biochip can comprise a nanopore in a membrane (e.g., lipid bilayer) adjacent or in proximity to an electrode. Methods are described for forming the membrane and inserting the nanopore into the membrane. The biochips and methods can be used for nucleic acid (e.g., DNA) sequencing. The present disclosure also describes methods for detecting, sorting, and binning molecules (e.g., proteins) using biochips.

主权项

1. A method for forming a lipid bilayer for use in a nanopore sensor, comprising:
(a) directing a buffer solution in flow channel comprising an electrode having a material layer thereon, wherein said buffer solution is electrically conductive, and wherein said material layer comprises one or more lipids; (b) bringing said buffer solution in contact with said material layer; (c) measuring a current through said electrode to determine if at least a portion of said material layer has formed a lipid bilayer adjacent to said electrode; and (d) based on the determination of (c), applying a stimulus to said electrode to induce said at least the portion of the material layer to form said lipid bilayer adjacent to said electrode.