| 发明名称 |
PMT2-, OCH1-, PMT5- MUTANT CELLS |
| 摘要 |
The presented invention relates to the gene knockouts of the Pichia pastoris PMT2 gene in the och1-glycoengineered strain backgrounds to obtain recombinant proteins reduced amounts of O-linked glycosylation. Triple mutant, pmt2, pmt5, och1 strains are also part of the present invention. Method for making such strains and for producing heterologous polypeptides in such strains are also included in the present invention. |
| 申请公布号 |
US2015337274(A1) |
申请公布日期 |
2015.11.26 |
| 申请号 |
US201314652964 |
申请日期 |
2013.12.13 |
| 申请人 |
CHEN Ming-Tang;CHOI Byung-Kwon;DAVIDSON Robert;MERCK SHARP & DOHME CORP. |
发明人 |
Chen Ming-Tang;Choi Byung-Kwon;Davidson Robert |
| 分类号 |
C12N9/12;C12P21/00 |
主分类号 |
C12N9/12 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. An isolated fungal or lower eukaryotic host cell wherein said cell does not express functional PMT2 polypeptide, does not express functional OCH1 polypeptide; and, optionally, does not express functional PNT5 polypeptide. |
| 地址 |
Lebanon NH US |
