| 摘要 |
Nucleic acid molecules and compositions comprising one or more nucleic acid sequences that encode a consensus filovirus immunogen including a consensus Marburgvirus filovirus glycoprotein MARV GP immunogen, a consensus Ebolavirus Sudan filovirus glycoprotein SEBOV GP immunogen and a consensus Ebolavirus Zaire glycoprotein ZEBOV GP immunogen are disclosed. The coding sequences optionally include operable linked coding sequence that encode a signal peptide. Immunomodulatory methods and methods of inducing an immune response against filovirus, particularly Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire are disclosed. Method of preventing filovirus infection, particularly infection by Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire and methods of treating individuals infected with filovirus infection, particularly infection by Marburgvirus, Ebolavirus Sudan and Ebolavirus Zaire are disclosed. Consensus filovirus proteins are disclosed. |
| 主权项 |
1. A composition comprising:
a) a nucleic acid sequence that encodes a consensus Zaire ebolavirus envelope glycoprotein immunogen, the amino acid sequence of the consensus Zaire ebolavirus envelope glycoprotein immunogen selected from the group consisting of: SEQ ID NO:1, a fragment of SEQ ID NO:1 that comprises 600 or more amino acids, an amino acid sequence that is 95% homologous to SEQ ID NO:1, a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:1 that comprises 600 or more amino acids; SEQ ID NO:1 linked to an IgE signal peptide, a fragment of SEQ ID NO:1 that comprises 600 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is 95% homologous to SEQ ID NO:1 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:1 that comprises 600 or more amino acids linked to an IgE signal peptide; b) a nucleic acid sequence that encodes a consensus Sudan ebolavirus envelope glycoprotein immunogen, the amino acid sequence of the consensus Sudan ebolavirus envelope glycoprotein selected from the group consisting of: SEQ ID NO:2, a fragment of SEQ ID NO:2 that comprises 600 or more amino acids, an amino acid sequence that is 95% homologous to SEQ ID NO:2, a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:2 that comprises 600 or more amino acids, SEQ ID NO:2 linked to an IgE signal peptide, a fragment of SEQ ID NO:2 that comprises 600 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is 95% homologous to SEQ ID NO:2 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:2 that comprises 600 or more amino acids linked to an IgE signal peptide; and c) a nucleic acid sequence that encodes a Marburg marburgvirus Angola 2005 envelope glycoprotein immunogen, the amino acid sequence of the Marburg marburgvirus Angola 2005 envelope glycoprotein immunogen selected from the group consisting of: SEQ ID NO:3, a fragment of SEQ ID NO:3 that comprises 600 or more amino acids, an amino acid sequence that is 95% homologous to SEQ ID NO:3, a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:3 that comprises 600 or more amino acids, SEQ ID NO:3 linked to an IgE signal peptide, a fragment of SEQ ID NO:3 that comprises 600 or more amino acids linked to an IgE signal peptide, an amino acid sequence that is 95% homologous to SEQ ID NO:3 linked to an IgE signal peptide, and a fragment of an amino acid sequence that is 95% homologous to SEQ ID NO:3 that comprises 600 or more amino acids linked to an IgE signal peptide. |
